How To Remove Urine Smell From Mattress. To apply the baking soda sprinkle liberally on the surface of the mattress making sure that it covers both the stains and outside the edges of it. Let the vinegar penetrate the fibers of your mattress and leave it for 10 20 minutes to really ensure you remove that nasty urine smell.

Removing pet urine smell from a sofa or a mattress or carpeting is much easier with an enzyme cleaner. Use vinegar on the stain vinegar is not dangerous or toxic but it is a bit stinky. Vinegar is ideal for removing unpleasant odors and as a homemade stain remover for carpet and from all kinds of other surfaces too.
Vacuum to remove left over baking soda and solution residue.
Use vinegar on the stain vinegar is not dangerous or toxic but it is a bit stinky. Then dab the stain with the solution using a cloth and let it sit until it dries. It is also a natural disinfectant and will kill the microbes. Put undiluted white distilled vinegar in a spray bottle and dampen the stained area with it.